Yes too much masturbation can cause testicle pain.
Over masturbation depletes the body of necessary neurochemicals, which help power the sex drive and keep the body in a balanced state. These chemicals control everyday bodily functions such as hair growth; melatonin for sleep; thyroid chemicals to maintain weight; and acetylcholine for proper genital blood flow. Men who masturbate in excess see a depletion in blood flow and an increase of the inflammatory responses. When the testicles experience a reduction in blood flow along with inflammatory signals, pain can ensue.
Men who over masturbate see a decrease in testicular circulation and an increase in stagnation that leads to pain and testicle soreness. Meanwhile the testicles experience an increase in testicular interstitial tissue edema that creates groin pain.
The severity of testicular pain caused by over masturbation develops in stages. During the problematic stage, the sufferer experiences initial testicular discomfort. The severe stage causes lower back pain, while the addictive stage creates chronic fatigue.In the final, severely addictive stage, men experience all the aforementioned symptoms, including major testicular and groin pain.
Men who notice an increase in testicular pain after abusive masturbation may too experience other symptoms, including back pain, irritability and fatigue. If you notice your testicular pain associated with these symptoms, you may indeed be suffering from a hormone imbalance/reduced testicle blood flow.